Chromatography has a great impact o

Chromatography has a great impact on all areas of

analysis and, therefore, on the progress of science

in general. Chromatography differs from other methods of separation in that a wide variety of materials,

equipment, and techniques can be used. [Readers are

referred to references (1–19) for general and specific

information on chromatography.]. This chapter will

focus on the principles of chromatography, mainly

liquid chromatography (LC). Detailed principles and

applications of gas chromatography (GC) will be

discussed in Chap. 29. In view of its widespread

use and applications, high-performance liquid chromatography (HPLC) will be discussed in a separate

chapter (Chap. 28). The general principles of extraction are first described as a basis for understanding

chromatography.