Therefore, to make 1000 mL of 1X buffer, add 100 mL of 10X buffer stock to 900 mL of distilled water (1000 mL 100 mL contributed by the 10X buffer stock 900 mL).
Therefore, to make 1000 mL of 1X buffer, add 100 mL of 10X buffer stock to 900 mL of distilled water (1000 mL 100 mL contributed by the 10X buffer stock 900 mL).