To characterize the expression profiles of human iPSC-LBs and tocompare with those of a corresponding developmental stage, we carriedout microarray analysis of 83 selected genes that are serially upregulatedduring liver development. Hierarchical clustering analyses suggestedthat the expression profiles of human iPSC-LBs at day 4 of cultureresembled those of mouse E10.5 and E11.5 liver buds rather thanadvanced fetal or adult livers (Fig. 2d). These expression profiles wererelatively similar to those of human fetal liver cell-derived liver buds(FLC-LBs) (Fig. 2d), which also have an ability to form LBs (SupplementaryFigs 2a and 4).
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