The expression levels of ISA1 and ISA2 were substantially higher at high temperature than those at low temperature over the whole sampling period, indicting that ISA1 and ISA2 genes obviously exhibited an upregulation expression pattern at their RNA transcriptional levels when rice plants grew at high temperature (Figure 5A-D). On the other hand, the distinct expression pattern was observed for the responses of ISA3 and PUL to high temperature, with higher levels of transcriptional expression induced at the earlier filling stage and lower expression at the later filling stage relative to low temperature (Figures 5E-F and 6A-B).
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