There are several techniques to detect ROS. These include the direct and indirectmethods (Table 13.2). Direct methods include cytochrome c reduction, electron spinresonance, and nitroblue tetrazolium technique (NBT, and xyenol orange-basedassay and flow cytometry [7, 22] ). Indirect methods of measurement include theEndtz test, redox potential (GSH/GSSG), measurement of lipid peroxidation products levels, chemokines, measurement of oxidative DNA damage, and measurementof reactive nitrogen species by Greiss reaction and fluorescence spectroscopy [7
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