Only a small number of samples showedsigns of inhibition and a one in ten dilution was sufficientto remove the inhibition. The freezing/thawing of the samples could have destroyed some inhibitors of the PCR. It ismore likely that the sample was false-negative because ofthe low number of S. aureus present (10 CFU/ml). However, the real-time PCR assay could be further improved bythe inclusion of an internal amplification control to facilitatethe check for inhibition
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