Human liver-bud formation is initiated on the third or fourth week ofgestation, and this corresponds to embryonic day 9.5 (E9.5) to E10.5 formouse liver-bud formation. Similar to E10.5 mouse liver bud, immunohistochemistryshowed that human iPSC-LB is composed of proliferatingAFP-positive hepatoblasts as well as mesenchymal and endothelial progenitors (Fig. 2a)
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