. These in vivo and in vitro radiolabeling stud-ies collectively provide evidence for a behavioral and physiological sequence of events leading to feeding-induced de novo pheromone biosynthesis in male I. pini:(1) feeding on host phloem; (2) feeding-induced JH III release [i.e. biosynthesis (Feyereisen, 1985)] by the CA; and (3) JH III-stimulated de novo ipsdienol biosynthesis (Fig. 3).
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