GROWTEK bioreactor was used as modified solid-state fermentor to circumvent many of the problems associated with the conventional tray reactors for solidstate fermentation (SSF). Aspergillus oryzae IFO-30103 produced very high levels of a-amylase by modified solidstate fermentation (mSSF) compared to SSF carried out in enamel coated metallic trays utilizing wheat bran as substrate. High a-amylase yield of 15,833 U g–1 dry solid in mSSF were obtained when the fungus were cultivated at an initial pH of 6.0 at 32C for 54 h whereas a-amylase production in SSF reached its maxima (12,899 U g–1 dry solid ) at 30 C after 66 h of incubation. With the supplementation of 1% NaNO3, the maximum activity obtained was 19,665 U g–1 dry solid (24% higher than control) in mSSF, whereas, in SSF maximum activity was 15,480 U g–1 dry solid in presence of 0.1% Triton X-100 (20% higher than the control).
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