Spherical shape: Perfect sphere of the oocyte slows down permeation and equal distribution of cryoprotectant in the oocyte. This continuous concentration gradient results in longer exposure of oocyte to cryoprotectants, which may lead to toxic damage in one part of the oocyte offering less than optimal protection in the other. Therefore, the changes in the shape at the equilibration step due to osmotic effect may provide some type of benefits, but the cytoskeleton damage of theoocyte may also be triggered [11].
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