Polychrome methylene blue is methylene blue solution that has been allowed to oxidize by storing it exposed to the air (loosely plugged) for several months. A thin blood or exudate smear taken from a suspect case of anthrax is air-dried, flame-fixed and flooded with the stain for two to three minutes. The stained smear is washed and dried. The rods of B. anthracis stain blue and the capsular mate-rial a pale pink colour (Fig. 2.12). Any suspect anthrax material should be handled with care and the stained slides autoclaved after use. Viable spores may be present on the slide after staining
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