Light absorption by the microalgae increased by up to128% with increasing CO2 supply, while a reduction in the package effect meant that therewas less internal self-shading thereby increasing the efficiency of light absorption. CO2augmentation increased the maximum rate of both electron transport and photosynthesisby up to 256%. This led to increased biomass, with the highest yield occurring at the highestdissolved inorganic carbon/lowest pH combination tested (pH 6.5), with a doubling ofchlorophyll-a (Chl-a) biomass while total microalgal biovolume increased by 660% inMicractinium bornhemiense and by 260% in Pediastrum boryanum dominated cultures.Increased microalgal biomass did not off-set the reduction in ammonia volatilisation in thecontrol and overall nutrient removal was lower with CO2 than without. Microalgal nutrientremoval efficiency decreased as pH decreased and may have been related to decreased Chlaper cell. This experiment demonstrated that CO2 augmentation increased microalgalbiomass in two distinct communities, however, care must be taken when interpretingresults from standard biomass measurements with respect to CO2 augmentation.
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