Next, to recapitulate early organogenesis, human iPSC-HEs were cultivatedwith stromal cell populations; human umbilical vein endothelialcells (HUVECs) and human mesenchymal stem cells (MSCs) unless statedotherwise, because of their primitive nature (Supplementary Fig. 2aand Supplementary Discussion). Notably, although cells were plated in two-dimensional conditions, human iPSC-HEs self-organized intomacroscopically visible three-dimensional cell clusters by an intrinsicorganizing capacity up to 48 h after seeding (Fig. 1 b, c, Supplementary
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