The optimum conditions for the selected strain must be determined experimentally. This is
carried out in the laboratory, frequently in shake cultures. However, these have the disadvantage that their pH value and the concentration of dissolved oxygen cannot, as a rule, be controlled. Consequently, only the optimum temperature and composition of the nutrient solution and the supplementation of the substrate in them can be determined. The optimization of the pH and of the concentration of dissolved oxygen in the medium is generally carried out in small laboratory reactors which should be provided with a pH control and, if possible, with stirrer speed and gas flow measurement.
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