2.2. Samples preparation and Determination of histamineImmunoassay reagents, equipment and procedure: Screening analysis was performed using Neogen VERATOX ® Histamine kit. All reagents used for the ELISA screening analysis were furnished by Neogen Corporation. Samples preparations were carried out according to the kit instructions. A microtitre plate spectrophotometer (ELX -800, Bio-Tec U.S.A), was used for reading the ELISA plates at 620 nm. All of the analyses were performed in duplicate. The histamine concentration was determined by Neogen Veratox software v3.0.1.
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