This studyassessedtheeffectoftwopre

This studyassessedtheeffectoftwoprecursors(L-phenylalanine andp-amino
benzoic acid)usedaloneorincombinationwithmethyljasmonate,onthegrowth
and accumulationofpaclitaxel,baccatinIIIand10-deacetylbaccatinIIIinhairyroot
cultures of Taxusxmediavar.Hicksii. Thegreatestincreaseindrybiomasswas
observed after4weeksofculturinghairyrootsinmediumsupplementedwith1 mM of
L-phenylalanine (6.2gL1). Additionof1 mM of L-phenylalanine tothemediumalso
resulted inthegreatest10-deacetylbaccatinIIIaccumulation(422.7 mg L1), which
was notdetectedintheuntreatedcontrolculture.Supplementationwith100 mM of
L-phenylalanine togetherwith100 mM ofmethyljasmonateresultedinthe
enhancement ofpaclitaxelproductionfrom40.3 mg L1 (control untreatedculture)
to 568.2 mgL1, thehighestpaclitaxelcontentdetectedinthestudy.Theeffectofp-
amino benzoicacidontaxaneproductionwaslesspronounced,andthehighestyield
of paclitaxel(221.8 mgL1) wasobservedwhenthemediumwassupplementedwith
100 mM oftheprecursorincombinationwithmethyljasmonate.
Baccatin IIIwasnotdetectedundertheconditionsusedinthisexperimentandthe
investigated taxaneswerenotexcretedintothemedium.
& 2009 ElsevierGmbH.Allrightsreserved.

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This studyassessedtheeffectoftwoprecursors(L-phenylalanine andp-aminobenzoic acid)usedaloneorincombinationwithmethyljasmonate,onthegrowthand accumulationofpaclitaxel,baccatinIIIand10-deacetylbaccatinIIIinhairyrootcultures of Taxusxmediavar.Hicksii. Thegreatestincreaseindrybiomasswasobserved after4weeksofculturinghairyrootsinmediumsupplementedwith1 mM ofL-phenylalanine (6.2gL1). Additionof1 mM of L-phenylalanine tothemediumalsoresulted inthegreatest10-deacetylbaccatinIIIaccumulation(422.7 mg L1), whichwas notdetectedintheuntreatedcontrolculture.Supplementationwith100 mM ofL-phenylalanine togetherwith100 mM ofmethyljasmonateresultedintheenhancement ofpaclitaxelproductionfrom40.3 mg L1 (control untreatedculture)to 568.2 mgL1, thehighestpaclitaxelcontentdetectedinthestudy.Theeffectofp-amino benzoicacidontaxaneproductionwaslesspronounced,andthehighestyieldof paclitaxel(221.8 mgL1) wasobservedwhenthemediumwassupplementedwith100 mM oftheprecursorincombinationwithmethyljasmonate.Baccatin IIIwasnotdetectedundertheconditionsusedinthisexperimentandtheinvestigated taxaneswerenotexcretedintothemedium.& 2009 ElsevierGmbH.Allrightsreserved.

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Studyassessedtheeffectoftwoprecursors này (andp-amin L-phenylalanine
axit benzoic) usedaloneorincombinationwithmethyljasmonate, onthegrowth
và accumulationofpaclitaxel, baccatinIIIand10-deacetylbaccatinIIIinhairyroot
nền văn hóa của Taxusxmediavar.Hicksii. Thegreatestincreaseindrybiomasswas
quan sát after4weeksofculturinghairyrootsinmediumsupplementedwith1 mM của
L-phenylalanine (6.2gL? 1). Additionof1 mM của L-phenylalanine tothemediumalso
dẫn inthegreatest10-deacetylbaccatinIIIaccumulation (422,7 mg L? 1), mà
là notdetectedintheuntreatedcontrolculture.Supplementationwith100 mM của
L-phenylalanine togetherwith100 mM ofmethyljasmonateresultedinthe
nâng cao ofpaclitaxelproductionfrom40.3 mg L? 1 (điều khiển untreatedculture)
đến 568,2 MGL? 1, thehighestpaclitaxelcontentdetectedinthestudy.Theeffectofp-
amino benzoicacidontaxaneproductionwaslesspronounced, andthehighestyield
của paclitaxel (221,8 MGL? 1) wasobservedwhenthemediumwassupplementedwith
100 mM oftheprecursorincombinationwithmethyljasmonate.
Baccatin IIIwasnotdetectedundertheconditionsusedinthisexperimentandthe
tra taxaneswerenotexcretedintothemedium.
& 2009 ElsevierGmbH.Allrightsreserved.

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