Outline
6.1Introduction
6.2Historical perspective
Insight into the mode of DNA replication: the
Meselson–Stahl experiment
Insight into the mode of DNA replication:
visualization of replicating bacterial DNA
6.3DNA synthesis occurs from 5′ to 3′
6.4DNA polymerases are the enzymes that
catalyze DNA synthesis
Focus box 6.1 Bacterial DNA polymerases
6.5Semidiscontinuous DNA replication
Leading strand synthesis is continuous
Lagging strand synthesis is discontinuous
6.6Nuclear DNA replication in eukaryotic cells
Replication factories
Histone removal at the origins of replication
Prereplication complex formation at the origins of
replication
Replication licensing: DNA only replicates once per
cell cycle
Duplex unwinding at replication forks
RNA priming of leading strand and lagging strand
DNA synthesis
Polymerase switching
Elongation of leading strands and lagging strands
Proofreading
Maturation of nascent DNA strand
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