Ultrasound-assisted extraction was performed in an<br>ultrasound cleaning bath (Sonorex, Super RK 255H<br>type, 300 · 150 · 150 mm internal dimensions) by the<br>mode of the indirect sonication, at the fixed-frequency<br>of 35 kHz using as the working liquid. The temperature<br>of the sonicated bath was 25 C. The 500 mL sample<br>flask was charged with the batch and 50 mL of diethyl<br>ether was added. Sonication was held for 30 min. After<br>sonication the organic layer was transferred into a separation funnel and 20 mL of a saturated solution of NaCl<br>were added. The separated organic layer was collected.<br>The water layer was washed with another 20 mL portion<br>of the solvent. The whole organic extract was introduced<br>một lần nữa trong phễu tách và rửa sạch với 20 ml <br>của dung dịch bão hòa NaCl.
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