WhenC.gloeosporioidessporeswereincubatedinawater
layeronacoverglass,theydidnotgerminate.Additionof
GlcorSuedidnotenhancegerminationsignificantly.Yeast
extractcausednearcompletegerminationwithveryelongatedgermtubes(Fig.1).However,thegermtubesdidnot
differentiateintoappressoria.OnMilliporefilters,virtually
allofthesporesgerminatedwithoutanywax,butnoappressoriumformationwasobserved.Whenthesporeswere
placedoncoverglassescoatedwithavocadowax,mostspores
germinated,andvirtuallyallofthegermtubesformedappressoriawithin12h(Fig.1).Undertheseconditions,<1Mg
cm"
2
waxgavecompletegerminationandappressoriumformation.Whenconidiawereincubatedonanaqueouswax
suspensiononacoverglass,theformationofappressoria
wasalsoobserved.Theamountofwaxneededtostimulate
appressoriumformationinC.gloeosporioidesdependedon
themethodofapplication.Dose-dependencestudiesshowed
that50%appressoriuminductionrequired0.1^gofwax
cm"
2
whencoatedonacoverglassand0.001jugofwax
dispersedin100iiLofwaterandspreadona1-cm
2
cover
glass.Ontheotherhand,whensporeswereplacedonmoist
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