It was shown previously that vancomycin production,
but not growth, can be affected by the phosphate concentration
of the medium.In cultures where the
amino acids concentration was doubled at the start of
the incubation, there was a earlier depletion of phosphate
(Fig. 2A, B). If the concentration of phosphate
was increased from 2 to 5 mMsuch that the cultures were
no longer phosphate depleted, vancomycin production
could no longer be detected, confirming the negative
effect of phosphate seen in Plackett-Burman experiments.
The additional phosphate resulted in a 5% increase
in biomass levels (data not shown).
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