Growth performance (ADG, ADFI, and G:F) was determined for each interval from d 0 to 5, 5 to 11, and 0 to 11.One-half of the pigs (2 males and 2 females from each treatment) were euthanized on d 5 PI near the peakof infection, and the remainder were euthanized at theend of the experiment (d 11 PI) during recovery (Song etal., 2012). Before being euthanized, pigs were anesthe-tized by an intramuscular injection of a 1-mL combina-tion of telazol, ketamine, and xylazine (2:1:1) per 23 kgBW. The final mixture contained 100 mg telazol, 50 mg ketamine, and 50 mg xylazine in 1 mL (Fort Dodge Ani- mal Health, Fort Dodge, IA). After anesthesia, pigs were euthanized by intracardiac injection with 78 mg sodium pentobarbital (Sleepaway; Vortech Pharmaceuticals, Ltd., Dearborn, MI) per 1 kg of BW. Three 3-cm seg- ments from the middle of the jejunum, the ileum (close to the ileocecal junction), and the middle of the colon were collected and fixed in 10% neutral buffered forma- lin for histological analysis.
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