Summary of Method2.1 The MF method provides a direct count of bacteria in water based on the development of colonieson the surface of the membrane filter (Reference 18.5). A water sample is filtered through themembrane which retains the bacteria. Following filtration, the membrane containing the bacterialcells is placed on a selective medium, mEI agar, and incubated for 24 h at 41°C. All colonies(regardless of color) with a blue halo are recorded as enterococci colonies. Magnification and asmall fluorescent lamp are used for counting to give maximum visibility of colonies.
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