This studyassessedtheeffectoftwopre

This studyassessedtheeffectoftwoprecursors(L-phenylalanine andp-amino
benzoic acid)usedaloneorincombinationwithmethyljasmonate,onthegrowth
and accumulationofpaclitaxel,baccatinIIIand10-deacetylbaccatinIIIinhairyroot
cultures of Taxusxmediavar.Hicksii. Thegreatestincreaseindrybiomasswas
observed after4weeksofculturinghairyrootsinmediumsupplementedwith1 mM of
L-phenylalanine (6.2gL1). Additionof1 mM of L-phenylalanine tothemediumalso
resulted inthegreatest10-deacetylbaccatinIIIaccumulation(422.7 mg L1), which
was notdetectedintheuntreatedcontrolculture.Supplementationwith100 mM of
L-phenylalanine togetherwith100 mM ofmethyljasmonateresultedinthe
enhancement ofpaclitaxelproductionfrom40.3 mg L1 (control untreatedculture)
to 568.2 mgL1, thehighestpaclitaxelcontentdetectedinthestudy.Theeffectofp-
amino benzoicacidontaxaneproductionwaslesspronounced,andthehighestyield
of paclitaxel(221.8 mgL1) wasobservedwhenthemediumwassupplementedwith
100 mM oftheprecursorincombinationwithmethyljasmonate.
Baccatin IIIwasnotdetectedundertheconditionsusedinthisexperimentandthe
investigated taxaneswerenotexcretedintothemedium.
& 2009 ElsevierGmbH.Allrightsreserved.

Kết quả (Việt) 1:

Studyassessedtheeffectoftwoprecursors này (L-nmol amin andpaxit benzoic) usedaloneorincombinationwithmethyljasmonate, onthegrowthvà accumulationofpaclitaxel, baccatinIIIand10-deacetylbaccatinIIIinhairyrootnền văn hóa của Taxusxmediavar.Hicksii. Thegreatestincreaseindrybiomasswasquan sát after4weeksofculturinghairyrootsinmediumsupplementedwith1 mML-nmol (6.2gL 1). Additionof1 mM của L-nmol tothemediumalsokết quả inthegreatest10-deacetylbaccatinIIIaccumulation(422.7 mg L 1), màlà notdetectedintheuntreatedcontrolculture. Supplementationwith100 mML-nmol togetherwith100 mM ofmethyljasmonateresultedinthenâng cao ofpaclitaxelproductionfrom40.3 mg L 1 (kiểm soát untreatedculture)để 568.2 mgL 1, thehighestpaclitaxelcontentdetectedinthestudy. Theeffectofp-amin benzoicacidontaxaneproductionwaslesspronounced, andthehighestyieldcủa paclitaxel (221.8 mgL 1) wasobservedwhenthemediumwassupplementedwith100 mM oftheprecursorincombinationwithmethyljasmonate.Baccatin IIIwasnotdetectedundertheconditionsusedinthisexperimentandthetra taxaneswerenotexcretedintothemedium.& Năm 2009 ElsevierGmbH.Allrightsreserved.

Kết quả (Việt) 2:

Studyassessedtheeffectoftwoprecursors này (andp-amin L-phenylalanine
axit benzoic) usedaloneorincombinationwithmethyljasmonate, onthegrowth
và accumulationofpaclitaxel, baccatinIIIand10-deacetylbaccatinIIIinhairyroot
nền văn hóa của Taxusxmediavar.Hicksii. Thegreatestincreaseindrybiomasswas
quan sát after4weeksofculturinghairyrootsinmediumsupplementedwith1 mM của
L-phenylalanine (6.2gL? 1). Additionof1 mM của L-phenylalanine tothemediumalso
dẫn inthegreatest10-deacetylbaccatinIIIaccumulation (422,7 mg L? 1), mà
là notdetectedintheuntreatedcontrolculture.Supplementationwith100 mM của
L-phenylalanine togetherwith100 mM ofmethyljasmonateresultedinthe
nâng cao ofpaclitaxelproductionfrom40.3 mg L? 1 (điều khiển untreatedculture)
đến 568,2 MGL? 1, thehighestpaclitaxelcontentdetectedinthestudy.Theeffectofp-
amino benzoicacidontaxaneproductionwaslesspronounced, andthehighestyield
của paclitaxel (221,8 MGL? 1) wasobservedwhenthemediumwassupplementedwith
100 mM oftheprecursorincombinationwithmethyljasmonate.
Baccatin IIIwasnotdetectedundertheconditionsusedinthisexperimentandthe
tra taxaneswerenotexcretedintothemedium.
& 2009 ElsevierGmbH.Allrightsreserved.