Artificial Light Source Using Light

Artificial Light Source Using Light-emitting Diodes (LEDs) in the
Efficient Micropropagation of Spathiphyllum Plantlets
Duong Tan Nhut1,2, T. Takamura1
, H. Watanabe3
and M. Tanaka1
1
Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan
2
Dalat Institute of Biology, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam 3
Mitsubishi Chemical Corp., Yokohama Research Center, Yokohama 227-8520, Japan
Keywords: Acclimatization, in vitro growth, irradiation level
Abstract
Spathiphyllum plantlets were cultured in “Culture Pack”, a rockwool system,
with CO2 enrichment (3000 μmol mol-l) under different blue to red LED ratios and
different LED irradiation levels. Growth was compared to that under plant growth
fluorescent lamps (PGF) at 45 μmol m-2 s-1 PPF for 60 days. The highest total shoot
and root fresh weight of plantlets was obtained under 80% red + 20% blue LED as
compared to others. Total shoot and root fresh weight of in vitro plantlets grown
under 60 μmol m-2 s-1 was higher than that under 45, 75 μmol m-2 s-1 and PGF.
Attempts were also made to examine whether the light source during in vitro culture
affected the subsequent growth of plantlets 90 days after transferring to soil. The
fresh shoot and root weight of in vitro plantlets grown under 80 % red + 20% blue
LED was highest compared to others. Fresh shoot and root weights of in vitro
plantlets grown under 60 μmol m-2 s-1 and 75 μmol m-2 s-1 were higher than those
under 45 μmol m-2 s-1 and PGF. These results suggested that a culture system using
LED and film is advantageous for the micropropagation of Spathiphyllum plantlets