Strawberry is a member of the genus Fragaria in the Rosaceae family is a perennial, stoloniferous herb and popular fruit. Strawberry is a major berry crop around the world.It has been commercially cultivated in many counties in the world (Biswas et al., 2007). One of the important goals of the agricultural policy is to increase the acreage of strawberry to meet the demand of local fresh market, processing and export. The strawberry fruits are rich of vitamin C, B1, B2, protein, calcium, potassium, copper and iron, most of the nutritious elements essential for human being (Nehra et al., 1994). They are important in transformation genes and genetic engineering that genetic engineering of strawberry has already been reported by Jame et al. (1989), Nehra et al. (1990).Biotechnological approaches, especially genetic engineering, are an alternative efficient strategy to implement strawberry improvement.Importing mother plants is expensive. Healthy stocks used for propagation through conventional methods are not available. The in vitro culture of nodal cutting has been successful in the micropropagation of strawberry plants (Karhu and Hakala, 2002). It was have an opinion that several of plants can be produced within a year from a few mother plants by tissue culture technique (Boxus, 1983; Nehra, et al 1994). In this technique is useful if of the introduction of new cultivars. Propagation of strawberry is achieved either by runners or by in vitro micropropagation. The division of branches and
runners of strawberry are not always suitable for this type of cultivation
because of their emotional sensitivity of pathology agents (Dijkstra, 1993;
Swartz et al., 1981; Nehra, et al., 1994). Micropropagation of strawberry from
runners for beginning has been reported and may be refer to efficiently generate
a large number of disease free plants (Boxus, 1974). In addition, the storage of
tissue cultured propagules requires less space than traditional runner plant and
the in vitro storage can be initiated at any time during the production cycle
(Swartz et al., 1981). Micropropagated strawberry plant has been introduced to
prevent most of the plant and soil transmissible diseases.
This is the first time to report on in vitro propagation of strawberry in
iran. In the present examination of a simple protocol has been developed to
propagate strawberry through tissue culture methods from nodal segments.
Material and methods
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