The role of initial substrate concentration, iron oxide nanoparticles concentration and pH on hydrogen productionfrom distillery wastewater was well investigated in this batch study. Addition of iron oxide nanoparticles has shownenhancement effect on hydrogen production. The highest cumulative volume of hydrogen (380 ml), hydrogencontent (62.14%) and % COD reduction (72.5) was obtained under the optimal conditions (initial substrateconcentration of 110 g L-1 with nanoparticle concentration of 50 mg L-1 at pH 6).The comprehensive kinetic analysisof RH2, SHY, Rate of substrate utilization and biomass growth under different operational conditions (initialsubstrate concentration, iron oxide nanoparticle concentration and pH) was conducted. Kinetic analysis reveals thecorrelation between hydrogen production, substrate utilization and biomass growth. The high correlation coefficientindicated the suitability of the kinetic models to describe the experimental data satisfactorily
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