Gel filtration on Sepharose has also been used to study B serum proteins. Chromatographyon Sephadex G-25® essentially displays a first peak corresponding to the basic proteins, asecond to hevein, and a third to ergothioneine (see below) (Figure 5A). Filtration on SephadexG-75® applied to lutoids from immature latex reveals, in addition, a first peak correspondingto the microfibrils." 1 2 Separation of the basic protein peak above (G-25 or G-75) on acolumn of Sephadex G-50® makes it possible to distinguish three basic protein peaks (Figure5B). The first two peaks, which are quantitatively the most important, have been namedhevamines A and B. They correspond to a single cationic band in electrophoresis on cellulose69acetate, but separate into two closely spaced bands of approximately equal intensity in discelectrophoresis.
đang được dịch, vui lòng đợi..
