Analysis of residual nutrients from each steady
state showed that only those at the highest phosphate
concentration (8 mM) had no detectable amino acids
present, suggesting that, at this concentration of phosphate
growth, probably was nitrogen limited. This result
is supported by the decrease in qglucose and biomass concentration
at 8 mM phosphate and was confirmed by
pulsing the culture with additional amino acids and observing
an increase of 10% in biomass concentration
(data not shown)
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