Several key probe design features a

Several key probe design features are illustrated in
Figure 1A. Our labeling protocol adds a C residue to the
3' end of the miRNA. The inclusion of G residue at the
5' end increases the stability of binding to a labeled target
miRNA. Empirical probe selection studies have shown
that the incorporation of a 5' end hairpin provides valuable
discrimination for increasing target size specificity because
it destabilizes probe hybridization to larger, non-target RNAs.

Kết quả (Anh) 1:

Several key probe design features are illustrated inFigure 1A. Our labeling protocol adds a C residue to thethe 3 ' end of the miRNA. The inclusion of G residue at the5 ' end increases the stability of binding to a target labeledmiRNA. Empirical probe selection studies have shownthat the incorporation of a 5 ' end hairpin provides valuablediscrimination for increasing target size specificity becauseIt destabilizes water probe hybridization to larger, non-target RNAs.