On the report sheet, the tests are separated into groups of three, and a number (4, 2, or 1) is indicated for each. By adding the numbers corresponding to positive reactions within each group, a seven-digit profile number is obtained for the 20 tests of the API 20E strip.In some cases, the seven-digit profile is not discriminatory enough, and additional tests should be carried out, including the following: reduction of nitrates to nitrites (NO2), reduction of nitrites to N2 gas, motility, growth on MacConkey agar medium, oxidation of glucose, and fermentation of glucose.
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