Section 9: Parameter FlagsSection OverviewIntroduction The Swelab Alfa has several error and warning flags related to the measured parameters. These flags alert the operator of possible pathologic samples and parameter value errors.Contents This section contains the following topics:Topic See PageShort Description of Flags and Troubleshooting 60Detailed Description of Flags 62Flagging Capabilities 659.1 Short Description of Flags and TroubleshootingDescription The instrument has several parameter error flags related to the measured parameters. The flags are shown on the display and printouts. Resolving Error Flags The sample should be re-analyzed. If the problem persists see list below to identify the issue and/or refer to Section 12 Troubleshooting. Note Note that a parameter that is outside the “Normal Range”, refer to Section 9.4 for User Interface setup, is either marked with “H” or “L” on the printout and display to indicate if the value is higher or lower than the pre-set “Normal Range” values.Continued on next page Parameter FlagsFlag Cause ActionTU Blockage in aperture Run a “Prime cycle”, before re-analyzing the sample.TL Blockage in aperture Run a “Prime cycle”, before re-analyzing the sample.ST Counting time too short Run a “Prime cycle”, before re-analyzing the sample.TB Air bubbles present in system Run a “Prime cycle”, before re-analyzing the sample.OR • Air bubbles• Electrical disturbances• Incomplete lysing Re-analyze sampleSE • Clogging• Air bubbles• Electrical disturbances• Incomplete lysing Re-analyze sampleDE • Air bubbles• Electrical disturbances• Incomplete lysing• Incorrect gain settings• Pathological blood sample Re-analyze sampleFD Insufficient cell separation between RBC and PLT ranges Re-analyze sampleHS HGB readings vary too much Re-analyze sampleHH HGB light levels too high Run a “Prime cycle”, before re-analyzing the sample.HL HGB light levels too low Run a “Prime cycle”, before re-analyzing the sample.HO HGB offset level error Switch off the instrument and switch it back on after 3 seconds, and then re-analyze sampleHN HGB reading has a higher lightlevel than a “blank” Re-analyze sampleNM OM TM BD WBC differential abnormality Blood sample too old or pathological sampleAF Aspiration failure Re-analyze sampleDF Diluent pipette fill error Re-analyze sample(Verify instrument is filled)DP Diluent pipette emptying error Re-analyze sampleLF Lyse pipette fill error Re-analyze sampleLP Lyse pipette emptying error Re-analyze sampleTE Liquid transfer error Re-analyze sampleEC Expired control blood Use a fresh blood controlNR No Reagent Check reagent levelsER Expired Reagent Use a new lot of reagents Description This section covers the full description of the parameter warning and error flags that might occur. The list below is in order of the severity of the flag.
TU “Timeout upper detector” (RBC, PLT, WBC)
The liquid meniscus in the measuring tube passed the lower detector but never passed the upper one.
TL “Timeout lower detector” (RBC, PLT, WBC)
The liquid meniscus in the measuring tube never passed the lower detector.
ST “Short counting time error” (RBC, PLT, WBC)
The time for the liquid meniscus to pass from the lower to the upper detector is unreasonably short.
TB “Tube bubbles error” (RBC, PLT, WBC)
Air bubbles were detected by the start detector in the diluent column.
OR “Cell counting overrun error” (RBC, PLT, WBC)
The cell pulses arrived faster than the instrument could process them. Possible reasons might be air bubbles, electrical disturbances or incomplete lysing.
Note: Filtered away cell pulses might raise the OR flag, so it might not be possible to see them in the histograms or the result parameters. This is a hard limit determined by the software.
SE “Statistical error/flow rate variation error” (RBC, PLT, WBC)
The rate of cell pulses per time unit varies too much. Possible reasons might be clogging, air bubbles, electrical disturbances or difficult to lyse cells.
Note: Filtered away cells might raise the SE flag, so it might not be possible to see them in the histograms or the result parameters.
DE “Distribution error” (RBC, PLT, WBC)
The size distribution of the cell pulses departs from the expected one. Possible reasons might be air bubbles, electrical disturbances, difficult to lyse cells or an incorrect gain setting.
FD “Floating discriminator error” (PLT)
It was not possible to find the correct position for the floating RBC/PLT distribution curve. This flag often occurs on low PLT counts. The FD flag should only be reported if the corresponding parameter (PLT) value is high enough.
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HS “HGB statistical error” (HGB)
Individual HGB readings vary too much.
HH “HGB high level error” (HGB)
The HGB blank or sample readings reported a too high light level.
HL “HGB low level error” (HGB)
The HGB blank or sample readings reported a light lev
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