4. ConclusionsThe chitosan nanoparticles obtained showed spherical shapes and uniform sizes of approximately78 nm in the TEM micrographs. The stability of theCS-PMAA colloidal suspension was influenced bythe presence of the separated species from the urea,potassium chloride and calcium phosphate. TheFTIR results indicated the existence of electrostaticinteractions between –COO– and –NH3tosan nanoparticles and the elements N, P and Kpresent in the urea, calcium phosphate, potassiumchloride, respectively. The stability of the CS-PMAA colloidal suspension was higher with theaddition of nitrogen and potassium than with theaddition of phosphorus, due to the higher anioncharge from the calcium phosphate than the anioncharges from the potassium chloride and urea. Themean diameter increase of the CS-PMAA nanoparticles in suspension with the addition of differentcompounds indicated that the elements are beingaggregated on the surface of the chitosan nanoparticles. Further studies are needed to understand themechanism and to optimize the incorporation of theN, P and K elements into the CS-PMAA nanoparticles.
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