The bacterial culture grown on TSA slant agar was further analyzed by Gram staining andbiochemical analysis for identification of Vibrio spp. Gram staining was conducted for identificationof the morphology and properties of bacterial cell wall described as Gram positive or Gram negative.Kligler sugar iron was conducted for identification of Gram negative bacteria based on their ability toferment lactose, sucrose, dextrose as well as hydrogen sulphide (BD, Difco, Detroit, USA).Identification of the Vibrio spp. was based on the morphology and biochemical properties determinedby Gram staining and biochemical analys
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