Dual application of biomass for bio

Dual application of biomass for biohydrogen and biodiesel production could be considered a feasible
option for economic and sustainable energy production from microalgae. In this study, after a large
screening of fresh water microalgal isolates,Scenedesmussp. NBRI012 andChlorellasp. NBRI029 have
exhibited high biomass (1.31 ± 0.11 and 2.62 ± 0.13 g/L respectively) and lipid (244.44 ± 12.3 and
587.38 ± 20.2 mg/L respectively) yield with an organic carbon (acetate) source. Scenedesmus sp.
NBRI012 has shown the highest H2(maximum evolution of 17.72% v/v H2of total gases) production; it
produced H2continuously for seven days in sulfur-deprived TAP media. Sulfur deprivation during the
H2 production was found to increase the lipid content (410.03 ± 18.5 mg/L) of the residual biomass.
Fatty acid profile of the lipid extracted from the residual biomass ofScenedesmussp. NBRI012 has showed
abundance of fatty acids with a carbon chain length of C16 and C18. Cetane number, iodine value, and
saponification value of biodiesel were found suitable according to the range given by the Indian standard
(IS 15607), Brazilian National Petroleum Agency (ANP255) and the European biodiesel standard
EN14214.
2015 Elsevier Ltd. All rights reserved

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Dual application of biomass for biohydrogen and biodiesel production could be considered a feasibleoption for economic and sustainable energy production from microalgae. In this study, after a largescreening of fresh water microalgal isolates,Scenedesmussp. NBRI012 andChlorellasp. NBRI029 haveexhibited high biomass (1.31 ± 0.11 and 2.62 ± 0.13 g/L respectively) and lipid (244.44 ± 12.3 and587.38 ± 20.2 mg/L respectively) yield with an organic carbon (acetate) source. Scenedesmus sp.NBRI012 has shown the highest H2(maximum evolution of 17.72% v/v H2of total gases) production; itproduced H2continuously for seven days in sulfur-deprived TAP media. Sulfur deprivation during theH2 production was found to increase the lipid content (410.03 ± 18.5 mg/L) of the residual biomass.Fatty acid profile of the lipid extracted from the residual biomass ofScenedesmussp. NBRI012 has showedabundance of fatty acids with a carbon chain length of C16 and C18. Cetane number, iodine value, andsaponification value of biodiesel were found suitable according to the range given by the Indian standard(IS 15607), Brazilian National Petroleum Agency (ANP255) and the European biodiesel standardEN14214.2015 Elsevier Ltd. All rights reserved

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