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Zymomonas mobilis genes for pyruvat
Zymomonas mobilis genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were
integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pif).
Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to
increase expression. Spontaneous mutants were selected for resistance to high levels of chloramphenicol that
also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of
alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the
previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations
of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of
conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the
additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced
to inactivate succinate production (frd) and to block homologous recombination (recA).
integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pif).
Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to
increase expression. Spontaneous mutants were selected for resistance to high levels of chloramphenicol that
also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of
alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the
previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations
of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of
conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the
additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced
to inactivate succinate production (frd) and to block homologous recombination (recA).
0/5000
Zymomonas mobilis genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were
integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pif).
Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to
increase expression. Spontaneous mutants were selected for resistance to high levels of chloramphenicol that
also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of
alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the
previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations
of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of
conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the
additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced
to inactivate succinate production (frd) and to block homologous recombination (recA).
integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pif).
Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to
increase expression. Spontaneous mutants were selected for resistance to high levels of chloramphenicol that
also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of
alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the
previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations
of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of
conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the
additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced
to inactivate succinate production (frd) and to block homologous recombination (recA).
đang được dịch, vui lòng đợi..
Zymomonas mobilis genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were
integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pif).
Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to
increase expression. Spontaneous mutants were selected for resistance to high levels of chloramphenicol that
also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of
alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the
previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations
of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of
conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the
additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced
to inactivate succinate production (frd) and to block homologous recombination (recA).
integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pif).
Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to
increase expression. Spontaneous mutants were selected for resistance to high levels of chloramphenicol that
also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of
alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the
previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations
of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of
conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the
additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced
to inactivate succinate production (frd) and to block homologous recombination (recA).
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