Vibrio harveyi and Vibrio parahaemolyticus, are opportunistic
pathogens that cause symptomatic infections when
the shrimp are stressed. V. parahaemolyticus is the dominant
species in shrimp affected by red disease and tail
necrosis (Jayasree et al., 2006). Most of the pathogenic
Vibrio sp. has the virulence characteristic like haemolytic
activity and proteolytic activity. Potentially, pathogenic V.
parahaemolyticus strains could be differentiated from the
related nonpathogenic marine vibrios based on haemolytic
and non haemolytic activity. Moreover, extracellular
products secreted by the V. parahaemolyticus were found
to be highly toxic to tiger prawn (Sudheesh and Xu,
2001). However, gastroenteritis caused by V. parahaemolyticus
has been associated with foods prepared with
seaweeds (Mahmud et al., 2007). In 2006, an outbreak of
V. parahaemolyticus infections resulted in 177 cases and
was linked to the consumption of contaminated raw
shellfish including oysters (Balter et al., 2006).
Identification and detection of total and pathogenic V.
parahaemolyticus by polymerase chain reaction (PCR)
have been extensively studied. A toxin regulatory gene
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