Hairy root cultures are an attractive experimental system asthey are long-term aseptic root clones, genetically stable, withhigh growth rate compared to those of the fastest growing cellsuspensions, and also for the development of transformed plants.Hairy roots are amenable for scale-up in bioreactors and arewidely preferred for the production of various chemical substancesthat are analogous to root systems. The neoplastic roots,produced by A. rhizogenes infection, characterized by highgrowth rate are demonstrated to produce higher levels of secondarymetabolites or amounts comparable to that of intactplants (Romero et al. 2009; Rostampour et al. 2009; Shinde et al.2009). In Plumbago spp. roots are the major source of plumbagin.The production of plumbagin by several plumbagin-yieldingplants through in vitro culture of Plumbago zeylanica (Heble etal. 1974), P. rosea (Panichayupakaranant and Tewtrakul 2002),Drosophyllum lusitanicum (Nahálka et al. 1996, 1998), Droseranatalensis, D. capensis (Crouch et al. 1990), D. gigantea(Budzianowski 2000), and Diospyros olen (Evans et al. 1999)has been accomplished. Of the different plumbagin-yieldingspecies, P. indica occupies the first pl
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