CHYMOSIN CONCENTRATIONChymosin concentration affects gel firmness (Lom-holt and Qvist 1999), and enzyme kinetics havebeen used to study these effects. An increase in thechymosin concentration (larger amounts of rennetadded to milk) reduces the total time required forrennet clotting, measured by the appearance of thefirst curd particle. As a result, the secondary phaseof rennet action will also proceed much earlier, withthe net result of an increase in the rate of increase ingel firmness. This property of chymosin is some-times used to control the rennet coagulation in con-centrated milks, which have a tendency to formfirmer curd because of the higher protein content.Lowering the amount of rennet reduces the rate ofcurd firming. It should also be noted that with analteration in the amount of rennet added to milk,there also will be a change in the amount of residualchymosin in the cheese.
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