In the present study, expression of GUS activity in the regenerated shoots was not directly correlated with kanamycin resistance. The lack of expression of the gus A gene in kanamycin resistant shoots may be due to the alteration or loss of gus A gene. Ying et al. (1992) usingthe same plasmid in Carthamus, and Ottaviani et al. (1993) in potato, found expression of gus A gene only in some of the kanamyein resistant calli.
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