Many modifications to this procedure have been advocated, and the following protocol is typical of themethod used in diagnostic laboratories. Less distortion of protozoan cysts occurs with this method thanwith zinc sulphate flotation. This method achieves a concentration of 15–50-fold, dependent on theparasite sought, and provides a good concentrate of protozoan cysts and helminth eggs, which arediagnostically satisfactory. All steps that can generate aerosols (excluding centrifugation) should beperformed in an operator protection safety cabinet.
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