2.5. Enzymatic hydrolysis of chitosan in the UF membrane reactorThe UF membrane reactor (Millipore Minitane system, Millipore Co., USA) is shown in Fig. 1. It consisted of a supplement tank, a reservoir tank, a water bath for controlling reaction temperature, three peristaltic pumps, a membrane cartridge with molecular weight cut off (MWCO) 3000 Da and an enzyme reactor vessel. The amount of reducing sugar produced from chitosan by the UF membrane reactor at a given permeation rate was compared with the optimum conditions for the enzyme concentration, incubation time, and reaction temperature determined in the batch reactor. The oligosaccharides produced from chitosan at different permeation rates were analyzed by HPLC on the TSK gel NH2-60 column. The reactor system for semi-continuous production of oligosaccharides was also operated under the optimum conditions. A new substrate was added to the reactor tank after enough incubation and recycling time had been allowed to hydrolyze the chitosan solution.
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