NanoDrop 2000/2000c Spectrophotomet

NanoDrop 2000/2000c Spectrophotometer
V1.0 User Manual
The inform
ation in this pu
b
lication i
s
pro
v
ided for refe
re
n
c
e only. All information
contai
ned in t
h
is pu
blication is
believed to b
e
corre
c
t and
compl
e
te. Th
ermo Fi
she
r
Scientif
ic shal
l not be liable
for errors co
ntained h
e
rei
n
nor for
incid
ental or
con
s
e
que
ntia
l damage
s in
con
n
e
c
tion wi
th t
he furnishi
ng, perfo
rma
n
ce o
r
use of this materi
al. All
prod
uct spe
c
i
f
ication
s
, as
well a
s
the informatio
n co
ntained
in thi
s
publi
c
ation, a
r
e su
bje
c
t to cha
nge
witho
u
t notice.
This pu
blication may conta
i
n or refe
ren
c
e informatio
n and
produ
cts
prote
c
ted by copyri
ghts o
r
patents a
nd d
oes n
o
t
convey any li
cense under our patent righ
ts, nor the ri
ghts of others. We do not
assume any liability arising out of
any infringe
m
ents of patent
s or ot
her rights
of third parties
.
We ma
ke no
warra
n
ty of any kind with regard to this mate
rial, in
cl
uding b
u
t not limited to the implied warra
n
ties of
mercha
ntabili
ty and fitness for a particul
a
r pu
rpo
s
e. Custom
e
r
s are
ultimately respon
sible for v
a
lidation of th
eir
sy
st
em
s.
© 2009 Th
ermo Fish
er Sci
entific Inc. A
ll rights reserv
ed. No pa
rt of this publi
c
ati
on may be st
ored in a
retri
e
val
system, tran
smitted, or rep
r
odu
ce
d in an
y way, incl
udi
ng but not limited to photoc
opy, photogra
ph, magneti
c
or
other record,
without ou
r
prior written permissi
on.
For Technic
a
l Support, p
l
ease con
t
ac
t:
Therm
o
Fish
er Scie
ntific
3411 Silversi
de Ro
ad
Bancroft Building, Suite 10
0
Wilmingto
n
, DE 1981
0 U.
S.A.
Teleph
one: 3
02-4
7
9
-
770
7
Fax: 302-7
9
2
-
715
5
E-mail: info@nano
dro
p
.co
m
www.na
nod
ro
p.com
For Internatio
nal Suppo
rt, plea
se
conta
c
t your lo
cal distrib
u
tor.
Microsoft, Windows, Wi
nd
ows NT and Excel are either trademarks
or regi
stered tradem
arks
of Microsoft
Corpo
r
ation i
n
the United
St
ates and/o
r
other countri
es.
Adobe an
d Acro
bat are tra
dema
r
ks of
Adobe System
s, Inco
rpo
r
ate
d
. All
other tradema
r
ks a
r
e
the prope
rty of
Therm
o
Fish
er Scie
ntific Inc. and its
su
bsidi
a
rie
s
.
Nan
o
Drop i
s
a tradem
ark of Therm
o
Fishe
r
Scie
ntific.
Revised 3/09
Table of Co
ntents
1.
INTR
OD
UCT
I
ON
...............................................................................................................................
.................................
1-1
I
NSTRUM
ENT
D
ESCRIPTIO
N
...............................................................................................................................
....................
1-1
I
NSTRUM
ENT
S
PECIFIC
A
T
I
ONS
...............................................................................................................................
................
1-1
Patents
...............................................................................................................................
................................................
1-1
S
AMPL
E
R
ET
EN
T
I
ON
P
ED
EST
A
L
M
EASUR
E
M
E
NTS
.................................................................................................................
1-2
Pedestal Basic
Use
...............................................................................................................................
.............................
1-2
C
UVE
TT
E
M
EAS
U
RE
ME
NT
S
...............................................................................................................................
.....................
1-3
Cuvet
t
e
Basi
c
Use
...............................................................................................................................
..............................
1-3
B
LANK
ING
AN
D
A
BSORBANC
E
C
AL
CU
LA
T
I
ON
S
.....................................................................................................................
1-4
F
LUORESC
E
NT
D
YES
...............................................................................................................................
..............................
1-5
2.
SOFT
WARE
...............................................................................................................................
..........................................
2-1
C
OMPU
TER
R
E
QUIRE
ME
NTS
...............................................................................................................................
...................
2-1
S
OFTWARE
I
NS
TAL
L
AT
ION
...............................................................................................................................
.....................
2-1
Regi
st
eri
n
g Yo
ur
I
n
st
r
u
m
e
nt
...............................................................................................................................
..............
2-2
S
OFTWARE
F
EA
T
U
RES
...............................................................................................................................
............................
2-2
Left Pa
ne
...............................................................................................................................
............................................
2-2
Ri
ght
Pa
ne
...............................................................................................................................
..........................................
2-5
D
ATA AN
D
A
CCOUNT
M
AN
A
G
EM
ENT
...............................................................................................................................
.....
2-7
My D
a
ta
...............................................................................................................................
.............................................
2-7
Reports
...............................................................................................................................
...............................................
2-7
A
u
t
o
sav
e
files
...............................................................................................................................
.....................................
2-9
O
p
tion
s
...............................................................................................................................
...............................................
2-9
3.
APPL
ICATIONS
...............................................................................................................................
...................................
3-1
O
VERVIE
W
...............................................................................................................................
.............................................
3-1
Q
UICK
S
TART
...............................................................................................................................
.........................................
3-1
M
EASUR
E
M
E
N
T
R
A
NGES
...............................................................................................................................
........................
3-2
N
UCLEIC
A
CID
...............................................................................................................................
.......................................
3-3
Ove
r
vi
ew
...............................................................................................................................
............................................
3-3
Nucleic Acid Calculations
...............................................................................................................................
.................
3-3
Uni
que
Sc
reen Features
...............................................................................................................................
.....................
3-4
Making
Nuclei
c Acid Measure
m
ents
...............................................................................................................................
.3
-
5
O
lig
o Calc
...............................................................................................................................
..........................................
3-6
M
ICRO
A
RRAY
...............................................................................................................................
.......................................
3-8
Ove
r
vi
ew
...............................................................................................................................
............................................
3-8
Dy
e/
Chr
o
m
opho
re E
d
i
t
o
r
...............................................................................................................................
...................
3-8
Uni
que
Sc
reen Features
...............................................................................................................................
.....................
3-8
Making Micro
Array Measure
m
ents
...............................................................................................................................
..
3-
9
O
lig
o Calc
...............................................................................................................................
........................................
3-10
UV
-V
IS
...............................................................................................................................
................................................
3-13
Ove
r
vi
ew
...............................................................................................................................
..........................................
3-13
Uni
que
Sc
reen Features
...............................................................................................................................
...................
3-13
Making UV-Vi
s
Measurem
ents
..................................................

NanoDrop 2000/2000c Spectrophotometer 
V1.0 User Manual 
The inform
ation in this pu
b
lication i
s
 pro
v
ided for refe
re
n
c
e only. All information 
contai
ned in t
h
is pu
blication is 
believed to b
e
 corre
c
t and 
compl
e
te. Th
ermo Fi
she
r
Scientif
ic shal
l not be liable
 for errors co
ntained h
e
rei
n
 nor for 
incid
ental or 
con
s
e
que
ntia
l damage
s in 
con
n
e
c
tion wi
th t
he furnishi
ng, perfo
rma
n
ce o
r
 use of this materi
al. All 
prod
uct spe
c
i
f
ication
s
, as 
well a
s
 the informatio
n co
ntained 
in thi
s
 publi
c
ation, a
r
e su
bje
c
t to cha
nge 
witho
u
t notice. 
This pu
blication may conta
i
n or refe
ren
c
e informatio
n and 
produ
cts 
prote
c
ted by copyri
ghts o
r
patents a
nd d
oes n
o
t 
convey any li
cense under our patent righ
ts, nor the ri
ghts of others. We do not
 assume any liability arising out of 
any infringe
m
ents of patent
s or ot
her rights
 of third parties
.
We ma
ke no 
warra
n
ty of any kind with regard to this mate
rial, in
cl
uding b
u
t not limited to the implied warra
n
ties of 
mercha
ntabili
ty and fitness for a particul
a
r pu
rpo
s
e. Custom
e
r
s are 
ultimately respon
sible for v
a
lidation of th
eir 
sy
st
em
s.
© 2009 Th
ermo Fish
er Sci
entific Inc. A
ll rights reserv
ed. No pa
rt of this publi
c
ati
on may be st
ored in a 
retri
e
val 
system, tran
smitted, or rep
r
odu
ce
d in an
y way, incl
udi
ng but not limited to photoc
opy, photogra
ph, magneti
c
 or 
other record, 
without ou
r 
prior written permissi
on. 
For Technic
a
l Support, p
l
ease con
t
ac
t: 
Therm
o
 Fish
er Scie
ntific 
3411 Silversi
de Ro
ad 
Bancroft Building, Suite 10
0 
Wilmingto
n
, DE 1981
0 U.
S.A. 
Teleph
one: 3
02-4
7
9
-
770
7
Fax: 302-7
9
2
-
715
5 
E-mail: info@nano
dro
p
.co
m
www.na
nod
ro
p.com
For Internatio
nal Suppo
rt, plea
se 
conta
c
t your lo
cal distrib
u
tor. 
Microsoft, Windows, Wi
nd
ows NT and Excel are either trademarks
 or regi
stered tradem
arks 
of Microsoft 
Corpo
r
ation i
n
 the United 
St
ates and/o
r
 other countri
es. 
Adobe an
d Acro
bat are tra
dema
r
ks of
 Adobe System
s, Inco
rpo
r
ate
d
. All 
other tradema
r
ks a
r
e
 the prope
rty of 
Therm
o
 Fish
er Scie
ntific Inc. and its 
su
bsidi
a
rie
s
. 
Nan
o
Drop i
s
 a tradem
ark of Therm
o
 Fishe
r
 Scie
ntific. 
Revised 3/09
Table of Co
ntents 
1.
INTR
OD
UCT
I
ON
...............................................................................................................................
.................................
1-1 
I
NSTRUM
ENT
D
ESCRIPTIO
N
...............................................................................................................................
....................
1-1 
I
NSTRUM
ENT
S
PECIFIC
A
T
I
ONS
...............................................................................................................................
................
1-1 
Patents
...............................................................................................................................
................................................
1-1 
S
AMPL
E 
R
ET
EN
T
I
ON
P
ED
EST
A
L 
M
EASUR
E
M
E
NTS
.................................................................................................................
1-2 
Pedestal Basic 
Use
...............................................................................................................................
.............................
1-2 
C
UVE
TT
E 
M
EAS
U
RE
ME
NT
S
...............................................................................................................................
.....................
1-3 
Cuvet
t
e
 Basi
c 
Use
...............................................................................................................................
..............................
1-3 
B
LANK
ING
 AN
D 
A
BSORBANC
E 
C
AL
CU
LA
T
I
ON
S
.....................................................................................................................
1-4 
F
LUORESC
E
NT
D
YES
...............................................................................................................................
..............................
1-5 
2.
SOFT
WARE
...............................................................................................................................
..........................................
2-1 
C
OMPU
TER
R
E
QUIRE
ME
NTS
...............................................................................................................................
...................
2-1 
S
OFTWARE 
I
NS
TAL
L
AT
ION
...............................................................................................................................
.....................
2-1 
Regi
st
eri
n
g Yo
ur
I
n
st
r
u
m
e
nt
...............................................................................................................................
..............
2-2 
S
OFTWARE 
F
EA
T
U
RES
...............................................................................................................................
............................
2-2 
Left Pa
ne
...............................................................................................................................
............................................
2-2 
Ri
ght
 Pa
ne
...............................................................................................................................
..........................................
2-5 
D
ATA AN
D 
A
CCOUNT
M
AN
A
G
EM
ENT
...............................................................................................................................
.....
2-7 
My D
a
ta
...............................................................................................................................
.............................................
2-7 
Reports
...............................................................................................................................
...............................................
2-7 
A
u
t
o
sav
e
 files
...............................................................................................................................
.....................................
2-9 
O
p
tion
s
...............................................................................................................................
...............................................
2-9 
3.
APPL
ICATIONS
...............................................................................................................................
...................................
3-1 
O
VERVIE
W
...............................................................................................................................
.............................................
3-1 
Q
UICK 
S
TART
...............................................................................................................................
.........................................
3-1 
M
EASUR
E
M
E
N
T
R
A
NGES
...............................................................................................................................
........................
3-2 
N
UCLEIC 
A
CID
...............................................................................................................................
.......................................
3-3 
Ove
r
vi
ew
...............................................................................................................................
............................................
3-3 
Nucleic Acid Calculations
...............................................................................................................................
.................
3-3 
Uni
que
 Sc
reen Features
...............................................................................................................................
.....................
3-4 
Making 
Nuclei
c Acid Measure
m
ents
...............................................................................................................................
.3
-
5
O
lig
o Calc
...............................................................................................................................
..........................................
3-6 
M
ICRO 
A
RRAY
...............................................................................................................................
.......................................
3-8 
Ove
r
vi
ew
...............................................................................................................................
............................................
3-8 
Dy
e/
Chr
o
m
opho
re E
d
i
t
o
r
...............................................................................................................................
...................
3-8 
Uni
que
 Sc
reen Features
...............................................................................................................................
.....................
3-8 
Making Micro 
Array Measure
m
ents
...............................................................................................................................
..
3-
9 
O
lig
o Calc
...............................................................................................................................
........................................
3-10 
UV
-V
IS
...............................................................................................................................
................................................
3-13 
Ove
r
vi
ew
...............................................................................................................................
..........................................
3-13 
Uni
que
 Sc
reen Features
...............................................................................................................................
...................
3-13 
Making UV-Vi
s
Measurem
ents
..................................................

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NanoDrop 2000/2000c Spectrophotometer V1.0 User Manual The information in this publication is provided for reference only. All information contained in this publication is believed to be correct and complete. Thermo FisherScientific shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing, performance or use of this material. All product specifications, as well as the information contained in this publication, are subject to change without notice. This publication may contain or reference information and products protected by copyrights orpatents and does not convey any license under our patent rights, nor the rights of others. We do not assume any liability arising out of any infringements of patents or other rights of third parties.We make no warranty of any kind with regard to this material, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Customers are ultimately responsible for validation of their systems.© 2009 Thermo Fisher Scientific Inc. All rights reserved. No part of this publication may be stored in a retrieval system, transmitted, or reproduced in any way, including but not limited to photocopy, photograph, magnetic or other record, without our prior written permission. For Technical Support, please contact: Thermo Fisher Scientific 3411 Silverside Road Bancroft Building, Suite 100 Wilmington, DE 19810 U.S.A. Telephone: 302-479-7707Fax: 302-792-7155 E-mail: info@nanodrop.comwww.nanodrop.comFor International Support, please contact your local distributor. Microsoft, Windows, Windows NT and Excel are either trademarks or registered trademarks of Microsoft Corporation in the United States and/or other countries. Adobe and Acrobat are trademarks of Adobe Systems, Incorporated. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. NanoDrop is a trademark of Thermo Fisher Scientific. Revised 3/09Table of Contents 1.INTRODUCTION................................................................................................................................................................1-1 INSTRUMENTDESCRIPTION...................................................................................................................................................1-1 INSTRUMENTSPECIFICATIONS...............................................................................................................................................1-1 Patents...............................................................................................................................................................................1-1 SAMPLE RETENTIONPEDESTAL MEASUREMENTS.................................................................................................................1-2 Pedestal Basic Use............................................................................................................................................................1-2 CUVETTE MEASUREMENTS....................................................................................................................................................1-3 Cuvette Basic Use.............................................................................................................................................................1-3 BLANKING AND ABSORBANCE CALCULATIONS.....................................................................................................................1-4 FLUORESCENTDYES.............................................................................................................................................................1-5 2.SOFTWARE.........................................................................................................................................................................2-1 COMPUTERREQUIREMENTS..................................................................................................................................................2-1 SOFTWARE INSTALLATION....................................................................................................................................................2-1 Registering YourInstrument.............................................................................................................................................2-2 SOFTWARE FEATURES...........................................................................................................................................................2-2 Left Pane...........................................................................................................................................................................2-2 Right Pane.........................................................................................................................................................................2-5 DATA AND ACCOUNTMANAGEMENT....................................................................................................................................2-7 My Data............................................................................................................................................................................2-7 Reports..............................................................................................................................................................................2-7 Autosave files....................................................................................................................................................................2-9 Options..............................................................................................................................................................................2-9 3.APPLICATIONS..................................................................................................................................................................3-1 OVERVIEW............................................................................................................................................................................3-1 QUICK START........................................................................................................................................................................3-1 MEASUREMENTRANGES.......................................................................................................................................................3-2 NUCLEIC ACID......................................................................................................................................................................3-3 Overview...........................................................................................................................................................................3-3 Nucleic Acid Calculations................................................................................................................................................3-3 Unique Screen Features....................................................................................................................................................3-4 Making Nucleic Acid Measurements................................................................................................................................3-5Oligo Calc.........................................................................................................................................................................3-6 MICRO ARRAY......................................................................................................................................................................3-8 Overview...........................................................................................................................................................................3-8 Dye/Chromophore Editor..................................................................................................................................................3-8 Unique Screen Features....................................................................................................................................................3-8 Making Micro Array Measurements.................................................................................................................................3-9 Oligo Calc.......................................................................................................................................................................3-10 UV-VIS...............................................................................................................................................................................3-13 Overview.........................................................................................................................................................................3-13 Unique Screen Features..................................................................................................................................................3-13 Making UV-VisMeasurements..................................................

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NanoDrop 2000 / 2000c Spectrophotometer
Manual V1.0 tài
Các thông
ation trong pu này
b
lication i
s
pro
v
ided cho refe
tái
n
c
chỉ e. Tất cả thông tin
contai
định nghĩa trong t
h
là pu
blication được
cho là b
e
Corre
c
t và
compl
e
te. Th
ermo Fi
cô
r
scientif
ic shal
l không chịu trách nhiệm
cho các lỗi đồng
ntained h
e
rei
n
và cũng không cho
incid
ental hoặc
con
s
e
que
NTIA
l thiệt hại
s trong
con
n
đ
c
tion wi
th t
ông furnishi
ng, perfo
RMA
n
ce o
r
sử dụng lieäu này
al. Tất cả
sản
UCT SPE
c
i
f
ication
s
, như
cũng một
là
các thập thông tin
co n
ntained
trong thi
s
PUBLI
c
ation, một
r
e su
bje
c
t để cha
nge
witho
u
t thông báo.
pu này
blication thể conta
i
n hoặc refe
ren
c
e informatio
n và
produ
cts
prote
c
ted bởi copyri
ghts o
r
bằng sáng chế một
nd d
oes n
o
t
chuyển tải bất kỳ li
có bản quyền thuộc righ bằng sáng chế của chúng tôi
ts, cũng không phải ri
ghts của người khác. Chúng tôi không
chịu bất kỳ trách nhiệm pháp lý phát sinh từ
bất kỳ vi phạm
m
huynh của bằng sáng chế
s hoặc ot
quyền của
các bên thứ ba
.
Chúng tôi ma
ke không
warra
n
ty của bất cứ loại nào đối với người bạn đời này với
rial, trong
cl
uding b
u
t không giới hạn các warra ngụ ý
n
mối quan hệ của
mercha
ntabili
ty và tập thể dục cho một particul
một
r pu
RPO
s
e. Tuỳ
e
r
s là
cuối cùng respon
sible cho v
một
lidation của thứ
EIR
sy
st
em
s.
© 2009 Th
ermo Fish
er Sci
entific Inc. A
ll quyền Reserv
ed. Không pa
rt này PUBLI
c
ati
trên có thể st
ored trong một
retri
e
val
hệ thống, tran
smitted, hoặc đại diện
r
ODU
ce
d trong một
cách y, bao gồm
udi
ng nhưng không giới hạn photoc
ao chép, photogra
ph, từ trường
c
hay
kỷ lục khác,
mà không ou
r
permissi trước bằng văn bản
trên.
Đối với Technic
một
l Hỗ trợ, p
l
dễ dàng con
t
ac
t:
Therm
o
Cá
er SCIE
ntific
3411 Silversi
de Ro
quảng cáo
Building Bancroft, Suite 10
0
Wilmingto
n
, DE 1981
0 U.
SA
Teleph
một: 3
02-4
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