Incubation atmosphere• Normal atmosphere (aerobic) for most of thepathogenic bacteria and all the fungi 5–10% CO2:■ Actinobacillus pleuropneumoniae■ Actinomyces viscosus■ Brucella species■ Campylobacter jejuni and C. fetus (optimally 6%O2, 10% CO2, 84% N2)■ Dermatophilus congolensis (primary isolation)■ Francisella tularensis■ Haemophilus species■ Histophilus species■ Taylorella equigenitalis• Anaerobic:■ Actinomyces bovis■ Bacteroides species■ Clostridium species■ Actinobaculum suis■ Fusobacterium species■ Peptoniphilus species■ Brachyspira hyodysenteriae.Incubation temperature• 37°C: Most of the microorganisms pathogenic foranimals including the mycoplasmas and fungi thatcause systemic mycoses• 42°C:■ Campylobacter jejuni■ Brachyspira hyodysenteriae• 28°C–30°C Leptospira serovars• 25°C: The dermatophytes except Trichophytonverrucosum that tolerates 37°C• 4°C: For cold enrichment of Listeria monocytogenes(from brain samples) and Yersinia enterocolitica and Y.pseudotuberculosis (from faecal samples).Incubation time• 24–48 hours: most of the rapidly growingbacteria• 48–72 hours: the rapidly growing bacteria whenplated on selective media
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