Micrograph of epithelium lining the small intestine, (a) stained with H&E, and (b) stained withthe PAS reaction for glycoproteins. With H&E, basophilic cell nuclei are stained purple whilecytoplasm stains pink. Cell regions with abundant oligosaccharides on glycoproteins, such as theends of the cells at the lumen (L) or the scattered mucus-secreting goblet cells (G), are poorlystained. With PAS, however, cell staining is most intense at the lumen, where projectingmicrovilli have a prominent layer of glycoproteins at the lumen (L) and in the mucin-richsecretory granules of goblet cells. Cell surface glycoproteins and mucin are PAS-positivebecause of their high content of oligosaccharides and polysaccharides respectively. The PASstainedtissue was counterstained with hematoxylin to show the cell nuclei. Both X300.
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