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Materials. Pea protein isolate (85%
Materials. Pea protein isolate (85% protein content) was a gift
from Nutri-Pea Ltd. (Portage la Prairie, Manitoba, Canada). Renin
assay buffer, renin protein, and renin substrate were provided in the
Renin Inhibitor Screening Assay Kit purchased from Cayman (Cayman
Chemical, Ann Arbor, MI). Purified rabbit lung angiotensin I-converting
enzyme (ACE), thermolysin (type X, Bacillus thermoproteolyticus
rokko, 39 units/mg solid), hippuryl-histidyl-leucine, and
hippuric acid were purchased from Sigma (St. Louis, MO). HPLCgrade
acetonitrile was purchased from Fisher Scientific (Pittsburgh,
PA), and trifluoroacetic acid (TFA) was obtained from Fluka (Buchs,
Switzerland). HPLC-grade water generated by Milli-Q system (Millipore,
Bedford, MA, USA) was used for the preparation of the HPLC mobile
phase; all other chemicals were of reagent grade and obtained from
Sigma (St. Louis, MO).
from Nutri-Pea Ltd. (Portage la Prairie, Manitoba, Canada). Renin
assay buffer, renin protein, and renin substrate were provided in the
Renin Inhibitor Screening Assay Kit purchased from Cayman (Cayman
Chemical, Ann Arbor, MI). Purified rabbit lung angiotensin I-converting
enzyme (ACE), thermolysin (type X, Bacillus thermoproteolyticus
rokko, 39 units/mg solid), hippuryl-histidyl-leucine, and
hippuric acid were purchased from Sigma (St. Louis, MO). HPLCgrade
acetonitrile was purchased from Fisher Scientific (Pittsburgh,
PA), and trifluoroacetic acid (TFA) was obtained from Fluka (Buchs,
Switzerland). HPLC-grade water generated by Milli-Q system (Millipore,
Bedford, MA, USA) was used for the preparation of the HPLC mobile
phase; all other chemicals were of reagent grade and obtained from
Sigma (St. Louis, MO).
0/5000
Materials. Pea protein isolate (85% protein content) was a gift
from Nutri-Pea Ltd. (Portage la Prairie, Manitoba, Canada). Renin
assay buffer, renin protein, and renin substrate were provided in the
Renin Inhibitor Screening Assay Kit purchased from Cayman (Cayman
Chemical, Ann Arbor, MI). Purified rabbit lung angiotensin I-converting
enzyme (ACE), thermolysin (type X, Bacillus thermoproteolyticus
rokko, 39 units/mg solid), hippuryl-histidyl-leucine, and
hippuric acid were purchased from Sigma (St. Louis, MO). HPLCgrade
acetonitrile was purchased from Fisher Scientific (Pittsburgh,
PA), and trifluoroacetic acid (TFA) was obtained from Fluka (Buchs,
Switzerland). HPLC-grade water generated by Milli-Q system (Millipore,
Bedford, MA, USA) was used for the preparation of the HPLC mobile
phase; all other chemicals were of reagent grade and obtained from
Sigma (St. Louis, MO).
from Nutri-Pea Ltd. (Portage la Prairie, Manitoba, Canada). Renin
assay buffer, renin protein, and renin substrate were provided in the
Renin Inhibitor Screening Assay Kit purchased from Cayman (Cayman
Chemical, Ann Arbor, MI). Purified rabbit lung angiotensin I-converting
enzyme (ACE), thermolysin (type X, Bacillus thermoproteolyticus
rokko, 39 units/mg solid), hippuryl-histidyl-leucine, and
hippuric acid were purchased from Sigma (St. Louis, MO). HPLCgrade
acetonitrile was purchased from Fisher Scientific (Pittsburgh,
PA), and trifluoroacetic acid (TFA) was obtained from Fluka (Buchs,
Switzerland). HPLC-grade water generated by Milli-Q system (Millipore,
Bedford, MA, USA) was used for the preparation of the HPLC mobile
phase; all other chemicals were of reagent grade and obtained from
Sigma (St. Louis, MO).
đang được dịch, vui lòng đợi..
Materials. Pea protein isolate (85% protein content) was a gift
from Nutri-Pea Ltd. (Portage la Prairie, Manitoba, Canada). Renin
assay buffer, renin protein, and renin substrate were provided in the
Renin Inhibitor Screening Assay Kit purchased from Cayman (Cayman
Chemical, Ann Arbor, MI). Purified rabbit lung angiotensin I-converting
enzyme (ACE), thermolysin (type X, Bacillus thermoproteolyticus
rokko, 39 units/mg solid), hippuryl-histidyl-leucine, and
hippuric acid were purchased from Sigma (St. Louis, MO). HPLCgrade
acetonitrile was purchased from Fisher Scientific (Pittsburgh,
PA), and trifluoroacetic acid (TFA) was obtained from Fluka (Buchs,
Switzerland). HPLC-grade water generated by Milli-Q system (Millipore,
Bedford, MA, USA) was used for the preparation of the HPLC mobile
phase; all other chemicals were of reagent grade and obtained from
Sigma (St. Louis, MO).
from Nutri-Pea Ltd. (Portage la Prairie, Manitoba, Canada). Renin
assay buffer, renin protein, and renin substrate were provided in the
Renin Inhibitor Screening Assay Kit purchased from Cayman (Cayman
Chemical, Ann Arbor, MI). Purified rabbit lung angiotensin I-converting
enzyme (ACE), thermolysin (type X, Bacillus thermoproteolyticus
rokko, 39 units/mg solid), hippuryl-histidyl-leucine, and
hippuric acid were purchased from Sigma (St. Louis, MO). HPLCgrade
acetonitrile was purchased from Fisher Scientific (Pittsburgh,
PA), and trifluoroacetic acid (TFA) was obtained from Fluka (Buchs,
Switzerland). HPLC-grade water generated by Milli-Q system (Millipore,
Bedford, MA, USA) was used for the preparation of the HPLC mobile
phase; all other chemicals were of reagent grade and obtained from
Sigma (St. Louis, MO).
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