2) Chemical data recording—Periodically measure dissolved oxygen, pH, conductivity,
temperature, hardness, and alkalinity. Typically measure hardness, alkalinity, and conductivity at
least weekly. Record temperature at least daily or continuously in one chamber that is centrally
located in the row of exposure chambers. Measure dissolved oxygen and pH at least weekly in
each exposure chamber containing surviving fish.
3) Verification of exposure concentrations—Before and during the test verify exposure
concentrations of the test chemical. This will confirm actual versus nominal concentrations.
Initially, analyze concentration in each replicate test chamber. If consistent concentrations
are observed among replicates at each concentration, make subsequent measurements on
composite samples from each replicate. However, make other checks of diluter function to
ensure proper operating conditions.
It is not necessary that measured concentrations be within any specific percentage of the
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