duction of ASCs both in vitro and in pathological tissue settings has been well documented [12, 26, 28–30]. In terms of the enhanced vascular density seen in this study, paracrine mechanisms are probable functional mediators. Furthermore, with ASCs involved in neo-vascular bed establishment at the site of wound repair, there is likely also an autocrine loop functioning, with local ASCs producing angiogenic factors that act on themselves or neighboring ASCs in reestablishment of vascularization.CONCLUSIONIn this study, we have successfully applied human ASC-seeded SFCS as a cytoprosthetic hybrid for reconstructive support in a cutaneous wound healing model. ASC-SFCS supports the delivery and engraftment of stem cells, as well as differentiation into fibrovascular and epithelial components. ASC-SFCS holdspromise as a future delivery vehicle for stem cells in clinical reconstructive settings.ACKNOWLEDGMENTSWe thank Jackie Furr and Sherita Daniel of the University of Texas M.D. Anderson Cancer Center core histology research laboratory for expert assistance in preparing histological sections. This work was supported in part by Grant 543102 from the Alliance of Cardiovascular Researchers (to E.U.A.) and by American Heart Association Southeast Affiliate Award 0555331B (to Y.-H.S.).DISCLOSURE OF POTENTIAL CONFLICTSOF INTERESTThe authors indicate no potential conflicts of interest.
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