Streaking the agar platesThe object of plate streaking is to obtain isolated bacterialcolonies that are required for observing colonial morphology,antibiotic sensitivity testing and for biochemical identification.The quadrant streak method, using the wholeplate, is usually employed for most diagnostic specimens(Fig. 2.22).The plate is first labelled, on the agar side, with the typeof specimen, date of inoculation and reference number. Awaterproof marker pen is used and the writing should bekept as near to the edge of the plate as possible so thatafter incubation the bacterial colonies are not obscured.Two inoculating loops are used so that one can be coolingwhile the other is in use. The loops should be flamed (Fig.2.23) before starting and then after streaks 1, 2 and 3. Thenumber of times the loops are flamed will depend on the
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