The essential parts of a spectrophotometer include light source, monochromator,sample and detector. Light source can be one lamp or two lamps in aspectrophotometer. When a single lamp is used, it covers the whole wavelengthrange. If there are two lamps, one is for UV wavelength range and another one isfor visible light range. The sample background can be subtracted with twodifferent methods, single beam instruments and double beam instruments.There are a spectrometer and a photometer in a spectrophotometer. With aspectrometer the measuring wavelength of light is chosen. This is done with amonochromator. A photometer measures the intensity of light.
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