• Samples should be taken from the affected site(s) asearly as possible following the onset of clinical signs.This is particularly important in viral diseases asshedding of virus is usually maximal early in theinfection. This is also true of enteric bacterialpathogens.• It is useful to collect samples from clinical cases andin-contact animals, particularly if there has beenan outbreak of disease. In-contact animals maybe at an earlier stage in the infection with a greaterchance of them shedding substantial numbers ofmicroorganisms.• Samples should be obtained from the edge of lesionsand some macroscopically normal tissue included.Microbial replication will be most active at thelesion’s edge.• It is important to collect specimens as aseptically aspossible, otherwise the relevant pathogen may beovergrown by numerous contaminating bacteria
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